Gabriel Virella Win

CLINICAL CHEMISTRY, Vol. 24, No. 3, 1978 515 Fig. 1. Results of an experiment designed to determine whether formation of Iysozyme-anti-lysozyme complexes changes the electrophoretic mobility of the enzyme (A, B) Stained electrophoresis and corresponding imprint of two samples of serum from a sheet immunized with human lgG. one of them having previously been incubated with lysozyme (S+ L). Notice that the lysozyme maintained a cathodai mobility identical to that of control lysozyme. (C, 1)) Stained electrophoresls and corresponding imprint of a 2 mg/mi solution of lysozyme (control). (E, F) Stained separation and corresponding imprint of lysozyme incubated with serum from a sheep immunized with the enzyme (S + L) and of a control serum sample from the same sheep (5). Notice that a substantial portion of the enzyme appears to be bound to gamma globulin fractions of the sheep serum. The arrows point to the application points, identically placed in all cellulose acetate strips.